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Upregulation of Mechanosensitive Channel Piezo1 Involved in High Shear Stress-induced Pulmonary Hypertension
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  • Jiyuan Chen,
  • Jinrui Miao,
  • Jing Liao,
  • Dansha Zhou,
  • Ziyi Wang,
  • Ziying Lin,
  • Yuqin Chen,
  • Chenting Zhang,
  • Xiaoyun Luo,
  • Yi Li,
  • Yue Xing,
  • Manjia Zhao,
  • Sophia Parmisano,
  • Haiyang Tang,
  • Jason X.-J. Yuan,
  • Kai Yang,
  • Dejun Sun,
  • Jian Wang
Jiyuan Chen
State Key Laboratory of Respiratory Disease

Corresponding Author:[email protected]

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Jinrui Miao
State Key Laboratory of Respiratory Disease
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Jing Liao
State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangdong Key Laboratory of Vascular Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University
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Dansha Zhou
First Affiliated Hospital of Guangzhou Medical University
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Ziyi Wang
State Key Laboratory of Respiratory Disease
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Ziying Lin
First Affiliated Hospital of Guangzhou Medical College
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Yuqin Chen
Guangzhou Medical University
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Chenting Zhang
First Affiliated Hospital of Guangzhou Medical College
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Xiaoyun Luo
State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University
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Yi Li
State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University
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Yue Xing
First Affiliated Hospital of Guangzhou Medical College
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Manjia Zhao
University of California San Diego
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Sophia Parmisano
University of California San Diego
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Haiyang Tang
State Key Laboratory of Respiratory Disease
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Jason X.-J. Yuan
Medicine Section of Physiology Division of Pulmonary, Critical Care and Sleep Medicine Department of Medicine
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Kai Yang
Guangzhou Medical University
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Dejun Sun
The People’s Hospital of Inner Mongolia
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Jian Wang
University of California San Diego
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Abstract

Background and Purpose: Piezo1 is a crucial mechanical sensitive channel involved in vascular remodeling. However, the role of Piezo1 in different types of vascular cells during the development of pulmonary hypertension (PH) induced by high flow is largely unknown. Experimental Approach: Based on previously established protocols, we established a rat PH model by left pulmonary artery ligation (LPAL) for 2 and 5 weeks to mimic the high flow and hemodynamic stress. Key Results: Results showed that right ventricular systolic pressure (RVSP) and right ventricular wall thickness were significantly increased in the LPAL groups compared with the SHAM group. Rats in LPAL-5w groups developed remarkable pulmonary vascular remodeling, resulting in decreased phenylephrine-induced contraction and acetylcholine-induced relaxation. On the one hand, in pulmonary arterial smooth muscle cells (PASMCs), upregulation of Piezo1 was observed in association with the elevation of [Ca2+]cyt in the PASMCs from both LPAL-2w and LPAL-5w groups versus respective SHAM groups. Notably, Piezo1 expression was directly upregulated by YAP/TEAD4. On the other hand, significantly upregulated Piezo1 expression was also presented in the lung tissues, mostly composed of pulmonary endothelial cells (ECs), from rats of LPAL-2w and -5ws groups, which can be transcriptionally regulated by RELA (p65) and contributes to the lung inflammation. Conclusion and Implications: Our results suggested the upregulation of Piezo1 in both PASMCs and ECs, coordinate together and contribute to the pulmonary vascular remodeling and dysfunction in LPAL-PH rats, providing novel insights into the cell type-specific effects of Piezo1 in the pulmonary vasculature during high flow-related PH.
Oct 2022Published in Thrombosis Research volume 218 on pages 52-63. 10.1016/j.thromres.2022.08.006