loading page

Bioengineered 3D-microfibrous-matrix modulates osteopontin release from MSCs and facilitates the expansion of hematopoietic stem cells
  • +5
  • Niji Nandakumar,
  • Malini Mohan,
  • Akhil Thilakan,
  • Hridhya Sidharthan,
  • Janarthanan R,
  • Deepti Sharma,
  • Shantikumar Nair,
  • Binulal Sathy
Niji Nandakumar
Amrita Vishwa Vidyapeetham Amrita Centre for Nanosciences and Molecular Medicine

Corresponding Author:[email protected]

Author Profile
Malini Mohan
Amrita Vishwa Vidyapeetham Amrita Centre for Nanosciences and Molecular Medicine
Author Profile
Akhil Thilakan
Amrita Vishwa Vidyapeetham Amrita Centre for Nanosciences and Molecular Medicine
Author Profile
Hridhya Sidharthan
Amrita Vishwa Vidyapeetham Amrita Centre for Nanosciences and Molecular Medicine
Author Profile
Janarthanan R
Amrita Institute of Medical Sciences and Research Centre
Author Profile
Deepti Sharma
Amrita Institute of Medical Sciences and Research Centre
Author Profile
Shantikumar Nair
Amrita Institute of Medical Sciences and Research Centre
Author Profile
Binulal Sathy
Amrita Vishwa Vidyapeetham Amrita Centre for Nanosciences and Molecular Medicine
Author Profile

Abstract

The osteopontin released from mesenchymal stem cells (MSC) undergoing lineage differentiation can negatively influence the expansion of hematopoietic stem cells (HSCs) in co-culture systems developed for expanding HSCs. Therefore, minimising the amount of osteopontin in the co-culture system is important for the successful ex vivo expansion of HSCs. Towards this goal, a bioengineered 3D-microfibrous matrix that can maintain MSCs in less osteopontin releasing condition has been developed and its influence on the expansion of HSCs has been studied. The newly developed 3D-matrix significantly decreased the release of osteopontin, depending on the MSC culture conditions used during the priming period before HSC seeding. The culture system with the lowest amount of osteopontin facilitated more than 40-fold increase in HSC number in 1 weeks’ time period. Interestingly, the viability of expanded cells and the CD34+ pure population of HSCs found to be the highest in the low osteopontin containing system. Therefore, bioengineered microfibrous 3D-matrices seeded with MSCs, primed under suitable culture conditions can be an improved ex vivo expansion system for HSC culture.
13 Jan 2022Submitted to Biotechnology and Bioengineering
13 Jan 2022Submission Checks Completed
13 Jan 2022Assigned to Editor
31 Jan 2022Reviewer(s) Assigned
09 Mar 2022Editorial Decision: Revise Major
09 Mar 2022Review(s) Completed, Editorial Evaluation Pending
07 May 20221st Revision Received
13 May 2022Submission Checks Completed
13 May 2022Assigned to Editor
13 May 2022Reviewer(s) Assigned
06 Jun 2022Review(s) Completed, Editorial Evaluation Pending
06 Jun 2022Editorial Decision: Revise Major
29 Jun 20222nd Revision Received
01 Jul 2022Submission Checks Completed
01 Jul 2022Assigned to Editor
05 Jul 2022Review(s) Completed, Editorial Evaluation Pending
05 Jul 2022Editorial Decision: Accept
Oct 2022Published in Biotechnology and Bioengineering volume 119 issue 10 on pages 2964-2978. 10.1002/bit.28175