Cloning, expression, purification, crystallization, data collection, structure solution and refinement
The FTT_1539 encoding gene from Francisella tularensis subsp. tularensis SCHU S4 strain (gi: 56708571; residues 1-476 was cloned [25], expressed [26], purified [27], set up for crystallization, screened, data was collected and structure was solved using the standard CSGID protocol, as described before [28] (see Supplementary Tables 1-5 with data on cloning and expression (Table S1), purification (Table S2), crystallization (Table S3), data collection (Table S4) and structure refinements (Table S5)). The structure was deposited to the Protein Data Bank (https://www.rcsb.org/) with the PDB code 4QVR.