Cloning, expression, purification, crystallization, data
collection, structure solution and refinement
The FTT_1539 encoding gene from Francisella tularensis subsp.
tularensis SCHU S4 strain (gi: 56708571; residues 1-476 was cloned
[25], expressed [26], purified [27], set up for
crystallization, screened, data was collected and structure was solved
using the standard CSGID protocol, as described before [28] (see
Supplementary Tables 1-5 with data on cloning and expression (Table S1),
purification (Table S2), crystallization (Table S3), data collection
(Table S4) and structure refinements (Table S5)). The structure was
deposited to the Protein Data Bank (https://www.rcsb.org/) with
the PDB code 4QVR.