Fig 2. Western blot and BN-PAGE analyses of peak I and II. (A) SDS-PAGE of peak I (lane 1) and peak II (lane 2) under DTT. (B) Western blot of peak I and peak II, with DTT, using Sup35 sequence-specific antibody. (C) BN-PAGE analysis of peak I. Peak I + DTT and Peak II + DTT all had the same amount of total protein in Figures 2A and 2B. The amount of protein in Figure 2C was twice as much as that in Figure 2A. Lanes M in Figures 2A and 2C: molecular weight protein marker comprising 116 kDa, 66.2 kDa, 45 kDa, 35 kDa, 25 kDa, 18.4 kDa, and 14.4 kDa.