Fig S1. pET28a-Sup35 vector map.
Fig S2. SEC curve of 14-day 45.4 min species in vitro.45.4 min species fractioned in Figure 3A were stored for 14 days at 4oC. The buffer used here were 20 mM Tris, pH 8.0, 1.2 M NaCl, and 10 mM DTT.