Fig 4. Long-term storage of Sup35 monomer. (A) SDD-AGE of
[psi- ] in vivo Sup35 monomer andE. coli 67.2 min monomer species stored for 14 days in
vitro . psi- and 67.2 min had the same amount of total proteins and 67.2
min species had more Sup35 proteins than psi- due to the background
expression in psi- cells. (B) SEC curve of 14-day 67.2 min speciesin vitro . E. coli Sup35 monomer in vitro was stored
for 14 days at 4 oC. The buffer used here was composed
of 20 mM Tris, pH 8.0, 1.2 M NaCl, and 10 mM DTT.