Immunofluorescence staining
Hela cells for immunofluorescence staining were plated on glass bottom
dishes. 24-48 hours after transfection, cells were fixed with pre-warmed
4% paraformaldehyde for 30 minutes at room temperature. Cells were
permeabilized with 0.3% Triton X-100 in TBST for 20 minutes at room
temperature, and then were blocked with 10% NGS in TBST for 1 hour at
room temperature. FOLR1 antibody (1:100, Proteintech, 23355-1-AP) was
diluted in blocking buffer and incubate overnight at 4℃. 14-16 hours
later, cells were rinsed with PBS and incubated with secondary antibody
(1:1000, Cell Signaling Technology, 8889S) diluted in blocking buffer
for 1 hour in the dark. Cells were rinsed with PBS and allowed to air
dry. A drop of antifade mounting medium with DAPI (Invitrogen, P36931)
was added to the slides which were covered with a coverslip. Images were
taken using a deconvolution microscope (Nikon T2).