Table 1. Examples of CRISPR-Cas-mediated approaches used in the study of root development.
Target gene Crop Promoter::Cas variant Root-associated parameters References
Involved in auxin-mediated root growth Involved in auxin-mediated root growth Involved in auxin-mediated root growth Involved in auxin-mediated root growth Involved in auxin-mediated root growth
OsPIN1a-OsPIN1d
rice
Cas9 (promoter not specified)
primary root length, crown root number, lateral root number, CRP number, root growth angle
OsAUX3 rice OsUbi::Cas9 lateral root number, primary root length, root hair length
OsAUX4 rice OsUbi::Cas9 primary root length, root hair length
OsTIR1, OsAFB2, OsAFB3, OsAFB4, OsAFB5 rice OsUbi::Cas9 primary root length, crown root number, lateral root number
OsIAA23 rice ZmUbi::Cas9 lateral root number, crown root number, primary root length, crown root length, total root weight
OsNAC2 rice 35S::Cas9 primary root length, crown root number
TAA1/FIB rice ZmUbi::Cas9 crown root number, lateral root number
DRO1, DRL1, DRL2 rice 2x35S::Cas9 root growth angle
Involved in cytokinin-mediated root growth Involved in cytokinin-mediated root growth Involved in cytokinin-mediated root growth Involved in cytokinin-mediated root growth Involved in cytokinin-mediated root growth
HK5, HK6 rice ZmUbi::Cas9 primary root length, lateral root number
RR21, RR22, RR23, RR24 rice ZmUbi10::Cas9 seminal root length, lateral root number
CKX1 barley ZmUbi::Cas9 total root length, total surface area, dry weight
CKX1, CKX3 barley ZmUbi::Cas9 total root length, total surface area, root mass, root diameter
Involved in abscisic acid-mediated root growth Involved in abscisic acid-mediated root growth Involved in abscisic acid-mediated root growth Involved in abscisic acid-mediated root growth Involved in abscisic acid-mediated root growth
OsABA8ox2 rice Ubi::Cas9 root elongation, root-to-shoot ratio
OsERA1 rice 2x35S::Cas9 primary root length
Involved in ethylene-mediated root growth Involved in ethylene-mediated root growth Involved in ethylene-mediated root growth Involved in ethylene-mediated root growth Involved in ethylene-mediated root growth
OsACS1, OsACS2 rice Ubi::Cas9 lateral root elongation in response to Pi deficiency
not specified not specified not specified not specified not specified
OsRopGEF3 rice OsUbi::Cas9 root hair length and width
OsLPR5 rice ZmUbi::Cas9 primary root length
EGT2 barley ZmUbi::SpCas9 seminal root angle, lateral root angle