3.5 R27 transfer does not correlate with recipient abundance, unlike pB10
The most abundant transconjugant ASVs carrying pB10 were also the most abundant ASVs in the recipient sewage community. In contrast, the most abundant transconjugant ASVs in sewage community/R27 were some of the low abundant ASVs in the recipient sewage community (figure 4a). Accordingly, a Pearson correlation test indeed indicated a positive correlation between the log10-transformed relative abundance of an ASV in the recipient sewage community and its corresponding log10-transformed relative abundance in sewage community/pB10 (R = 0.67, p <0.001), while showing no positive correlation between the log10-transformed relative abundance in the recipient sewage community and transconjugants ASVs in sewage community/R27 (R = -0.055, p = 0.66) (figure 5a).