Figure 4. Phylogeny and relative abundance of the
transconjugant sewage community. a) Phylogenetic tree, based
on Maximum Likelihood, for the 65 ASVs present (n taxa) in the
transconjugant sewage community after data cleaning. The ASVs are named
according to genus annotation. As the legends at the left indicate, ASVs
are colored according to family. ASVs were only assigned to
Gram-negative phylogroups indicated by Gram (+/-). The log10 relative
abundance of each ASV in the recipient sewage community (SC) is shown as
the white to blue scaled heatmap. A pseudo count of 1 read was added to
the OTU table before log transformation. The bar chart shows the
distribution of mean % relative abundance for each transconjugant
sewage community (pB10 and R27) between the ASVs, colored according to
the plasmid. Taxonomy of clades is indicated by the text on branches,
the phyla Proteobacteria, Bacteroides, the classes Flavobacteriia,
Gammaproteobacteria, Betaproteobacteria and orders Enterobacteriales
Pseudomonadales, Burkholderiales and Flavobacteriales. The bar plot b)
shows the mean % relative abundance (% rel. abundance on the y-axis)
between families in the two transconjugant sewage communities (Plasmid),
plotted on the x-axis. Colored according to families, this legend is
shared between figure a) and b). Error bars represent standard
deviation.