Figure 1. a) Shows the scenario of plasmid transfer from the red (mCherry) fluorescing E. coli donor strain to the sewage community (Recipient SC), where transconjugants will fluoresce green (GFP). b) Shows the scenario of plasmid transfer from the enriched transconjugant donor sewage community (Donor SC), fluorescing green (GFP) to the recipient E. coli strain fluorescing red (mCherry), where transconjugants will fluoresce both red (mCherry) and green (GFP). At the right hand side of both a) and b) is shown how the transconjugant will appear during flow cytometry. Created with BioRender.com