3.6 Culture enrichment of transconjugant sewage community resulted in single genera domination
Lastly, we assessed the taxonomy of the sorted and enriched transconjugant sewage community, in order to understand which hosts enable stable replication and maintenance of the two plasmids, and hence are able to support all three stages of plasmid host range. For all samples, enrichment promoted single-genus dominance, yet marked differences were observed between the two plasmid-associated sewage community transconjugant pools. While Serratia was primarily enriched in the transconjugant sewage community/R27 ( = 99.2%), Aeromonas dominated the transconjugant sewage community/pB10 pool (= 99%) (figure 5b).