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A test of rapid leaf photosynthesis-intercellular CO2 concentration response (RACiR) technique for evergreens and herbs
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  • Quanhong Lin,
  • Di Tian,
  • Changti Zhao,
  • Zhenyue Liu,
  • Bin Wang,
  • Zhengbing Yan,
  • Benjamin Stocker,
  • Yu'e Li,
  • Jingyun Fang
Quanhong Lin
Beijing Forestry University

Corresponding Author:[email protected]

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Di Tian
Beijing Forestry University
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Changti Zhao
Beijing Forestry University
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Zhenyue Liu
Beijing Forestry University
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Bin Wang
Chinese Academy of Agricultural Sciences
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Zhengbing Yan
University of Hong Kong
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Benjamin Stocker
ETH Zurich
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Yu'e Li
Chinese Academy of Agricultural Sciences
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Jingyun Fang
Peking University
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Abstract

Using traditional photosynthesis-intercellular CO2 concentration (A-Ci) response (TACiR) curves to obtain the maximum rates of ribulose-1,5-bisphosphate carboxylase oxygenase carboxylation (Vcmax) and electron transport (Jmax) is time-consuming and labor-intensive. Instead, the rapid A-Ci response (RACiR) technique provides a potential way with high efficiency. However, multiple parameter settings of RACiR technique for different plant life forms remain unclear. Here, we used Li-Cor 6800 to test the applicability and optimum parameter settings of RACiR curves for evergreens and herbs. We set 11 groups of [CO2], i.e., R1 (400-1500 ppm), R2 (400-200-800 ppm), R3 (420-20-620 ppm), R4 (420-20-820 ppm), R5 (420-20-1020 ppm), R6 (420-20-1220 ppm), R7 (420-20-1520 ppm), R8 (420-20-1820 ppm), R9 (450-50-650 ppm), R10 (650-50 ppm) and R11 (650-50-650 ppm), and made contrasts between TACiR and RACiR curves. We found that Vcmax and Jmax calculated by TACiR and RACiR overall showed no significant differences across 11 [CO2] gradients (P<0.05). The efficiency and accuracy of R2, R3, R4, R9 and R10 showed higher superiority than others. Moreover, the accuracy of manual empty chamber correction method was higher than the automatic method. In conclusion, the RACiR technique could be generally used to obtain photosynthetic parameters with higher efficiency than traditional methods for various life forms.