2.4 Microbial community analysis
Biofilm samples were also collected from the anodes of SAMFCs at the
stages of t0, t20 and
t100 by using a 10-mL syringe with a 0.9×150 mm
stainless steel needle for microbial community analysis. High-throughput
sequencing analysis based on all bacterial 16S rRNA was performed to
know the microbial communities present in the biofilms as described in
ref. (Chen et al., 2019) , detailed procedure see supplemental
information.