Figure Captions
Figure 1. Mean (±SE) (µg mg-1 of dried
weight) monthly variation of starch, total sugars, total non-structural
carbohydrates (NSC), and diterpenes of Pinus ponderosa trees in
six treatments from July to November 2014 and May to September 2015.
Figure 2. Means (±SE) (µg mg-1 of dried
weight) of starch, total sugars, and total non-structural carbohydrates
(NSCs) of Pinus ponderosa trees in six treatments. Trees included
in each comparison were reported in Table 1.
Figure 3. Monthly means
(±SE) (µg mg-1 of dried weight) of starch, total
sugars, and total non-structural carbohydrates (NSCs) of Pinus
ponderosa trees in different comparisons. * denotes that concentrations
vary in a given month (95% confidence level adjusted with Sidak method
for two estimates). Suppl. Table S4 shows statistical results.
Figure 4. Relationship between concentrations of total
diterpenes and non-structural carbohydrates (NSCs) of Pinus
ponderosa trees by treatments. Regression lines for: (a) NSC, (b) total
sugars, and (c) starch are from repeated measures ANCOVAs on the log of
total diterpenes. Top figures: Significance of interaction between NSCs
and total diterpenes were as follows: (a) Total NSCs:
F(5,256)=1.96, P=0.085, (b) Total sugars:
F(5,258)=2.18, P=0.057, and (c) Starch:
F(5,254)=1.34, P=0.247. Bottom figures. Significance of
interaction between NSCs and total monoterpenes were as follows: (d)
Total NSCs: F(5,255)=2.07, P=0.069, (e) Total sugars:
F(5,254)=1.58, P=0.166, (f) Starch:
F(5,286)=4.98, P=0.0002. Degrees of freedom were
calculated with Satterthwaite’s method.
Figure 5. Monthly means (±SE) (µg mg-1 of
dried weight) of carbohydrates, total diterpenes, and total monoterpenes
of dying and live Pinus ponderosa trees in the trenched-attacked
treatment. Data are from 2014. * shows months where differences among
treatments are significant (95% confidence level adjusted with Sidak
method for two estimates).
Figure 6. Monthly means (±SE) (µg mg-1 of
dried weight) of carbohydrates, total diterpenes, and total monoterpenes
of dying Pinus ponderosa trees in the trenched-attacked treatment
and live trees in the untrenched-control treatment. Data are from 2014.
* shows months where differences among treatments are significant (95%
confidence level adjusted with Sidak method for two estimates).
Figure 7. Relationship between carbohydrate and terpene
concentrations from 2014 for dying Pinus ponderosa trees in the
trenched-attacked category and all treatment categories of live trees
averaged over months. Solid (dying trees) and dashed (live trees) trend
lines indicate significant Pearson correlations. Dying trees n=8 and
live trees n=42 (except diterpene comparisons n=40). Total
non-structural carbohydrates (NSC) are the sums of starch and total
sugar concentrations.
Figure 8. Schematic representation of how mild drought alone or
in combination with pathogenic microbial infection and bark beetle
attacks affect carbohydrates and carbon-dependent defense metabolites
(terpenes) in pines.