Figure 1 The plasmid construct used for extracellular expression
of rAGL.
Figure 2 Fermentation curves of the recombinantP. pastoris in a 5-L fermenter, including optical density (▲),protein concentration (■), and enzyme activity (◆).
Figure 3 SDS-PAGE analysis of the extracellular rAGL expressed
in the engineered P. pastoris. Lane M, molecular weight markers;
Lane 1, culture supernatant; Lane 2, purified rAGL. The band
corresponding to rAGL is indicated with an arrow.
Figure 4 Effects of pH and temperature on the activity and
stability of rAGL .
Figure 5 Effect of metal ions on the activity of rAGL.
Figure 6 Influence of the factors including the reaction pH(A ), temperature (B) , maltose/AA·Na
molar ratio (C) and enzyme concentration (D) on AA-2G production.