Figure 13. EM images of lipoproteins formed by α-Syn mutant H50Q. The
region enclosed in the rectangle on the right is enlarged on the left.
The red and yellow circles have diameters of 3.8 and 5.6 nm predicted by
the tetramer and octamer models of Figs. 10 and 11 if the outer Ct
barrel is excluded. The gray and pink transparent rectangles over the
stack represent side views of octamer Nt-NAC and Ct domains, each pair
is 7.5 nm apart. (EM images were modified from Eichmann et al.
[19]).
The distance between discs is a relatively constant 7.5 nm, but
diameters of the discs vary. Each appears to be comprised of smaller
components, suggesting that the number of subassemblies per disc vary
(Fig 14a & b). Disc pairs that have approximately the same diameter
were grouped; image averaging with Adobe Photoshop was applied to each
group to obtain more detailed images (Fig. 14a). These averaged images
reveal vertical columns within and sometimes between the discs that are
~ 3.5 nm apart.