Figure 2. Target T1120, a DNA-binding protein DdrC (PDB: 7QVB).(A) a homodimer with two chains colored as cyan and green;(B) superposition of two chains showing the break point in the helix hA at residue LEU 125; (C) a Grishin plot showing the need for splitting (large angle between the data trend line and the diagonal). The plot was built on the GDT_TS results for all participating groups on the constituent domains D1: 8-125 and D2: 126-235 and the whole target in the chain A configuration.
Another example of such obligated splitting is target T1121, the Wadjet nuclease subunit JetD24. It is a homo-dimeric protein (Figure 3A) containing two domains that are flexibly linked and whose relative orientation differs in the two chains (Figure 3B). Because of that, the target was split into two EUs at the hinge point (residue 204). As in the previous case, such a split is strongly supported by the performance data (Figure 3C).