Flow Cytometry Analysis
Cells transfected with pEF-EGFP were analyzed 24 or 48 hours following
transfection to determine the percentage of transfected cells
(%EGFP+) and EGFP expression levels (mean EGFP) using
either a FACSCalibur Flow Cytometer (Figure 1) or a Millipore Sigma
Guava easyCyte HT sampling Flow Cytometer (Figures 2-6). Cells were
analyzed via side scatter analysis to differentiate live cell
populations from cell debris, while gates for fluorescent
(EGFP+) populations were defined using non-transfected
(EGFP-) control samples.