Identification of genes in common pathways using systems biology approach
Checking manually for the phenotypic relevance of the 64 genes yielded nine putative pathways encompassing 41 genes. This can be directly or indirectly linked to stuttering (table 1). Intriguingly, the possible candidate genes are different for the two families, but they fit into nine common pathways. Similarly, pathway browser tool in Reactome database (https://reactome.org) determine pathways that are over-represented. In this manner 25 most relevant pathways encompassing 14 genes (table 2), were sorted based on their p-value and the remaining genes were indicated as interactors (table A3). Thus the enriched gene list comprised of 14 genes that recurred in the two approaches (tables 1 and 2).