Headwater forest ecosystems of the western U.S. generate a large portion of the dissolved organic matter (DOM) transported across North America. Land cover changes that alter forest structure and forest species composition affect the quantity and composition of DOM transferred to aquatic ecosystems. Clear-cut harvesting effects ~1% of the forest area of North America annually, leaving most forests in varying stages of successional regrowth, and the total area of old-growth forest decreasing. The consequences of this widespread management practice on watershed carbon cycling remain unknown. We investigated the role of land cover change from old-growth subalpine forest to lodgepole pine dominated second-growth on the character and reactivity of DOM hillslope exports. We evaluated inputs of DOM from litter leachates and export of DOM collected at the base of trenched hillslopes during a three-year period (2016-2018) at the Fraser Experimental Forest in northcentral Colorado, USA. Dissolved organic carbon (DOC) and total dissolved nitrogen (TDN) were higher in lateral subsurface flow draining old- versus secondary-growth forest. Fluorescence spectroscopy showed that the DOM exported from the old-growth forest was more heterogeneous and aromatic and that proteinaceous, microbially processed DOM components were more prevalent in the second-growth forest. Biological oxygen demand (BOD) assays revealed much lower microbial metabolism of both DOM inputs from litter leachate and subsurface exports from old-growth forest. Old-growth and second-growth forests are co-mingled in managed ecosystems, and our findings demonstrate that the influence of species composition on DOM inputs can affect the reactivity of DOM transferred from terrestrial to aquatic ecosystems.