Figures
Figure 1. Schematic summary of the sample preparation methodology steps.
Figure 2: KAP sequences containing accessible cysteines identified in stage 1 (Bold), Step 2 (Bold underlined) and Step 3 (Bold Italicized).
Figure 3. Keratin type I sequences containing accessible cysteines identified in Step 1 (Bold), Step 2 (Bold underlined) and Step 3 (Bold Italicized), labelled cysteines are in red.
Figure 4. Keratin type II sequences containing accessible cysteines identified in Step 1 (Bold), Step 2 (Bold underlined) and Step 3 (Bold Italicized), labelled cysteines are in red.
Figure 5. A summary of the accessibility of cysteines in type I and II keratins. The two chains (I and II) are indicated by different shades of the same color. K31 and K85 are examples that demonstrate a general trend.
Figure 6. A schematic representation of the disulfide crosslinks between a pair of antiparallel molecules in the oxidized tetramer. The disulfide crosslinks are between cysteines at positions L1-9 and 1B-87 in type I keratins in the A11 alignment and two cysteines at position 2-90 in type I keratins in the A22 alignment. Two other cysteines (2-132 and T-5) thought to be involved in crosslinking are also marked on the diagram. The two chains (I and II) are indicated by different shades of the same color.