QCM analyses. 
Quartz Crystal Microbalance (QCM) measurements were carried out on Qsense D300 equipment with a Ti sensor (Q-Sense, Vastra Frolunda) that was intensively cleaned with a UV-ozone ProCleaner system (BioForce Nanosciences, Ames, IA) prior to use. Before measurements, the Ti-sensor and the chamber were equilibrated in 50 mM Tris-HCl (pH 8.0) and 150 mM NaCl (TBS) containing 0.05% polyoxyethylene sorbitan monolaurate (Tween 20) (TBST) at 25 °C. The proteins were prepared in a 2 mL volume at a concentration of 0.5 µM in TBST. The data were obtained and analyzed by Q soft 301 and q-tool (Q-Sense).