QCM analyses.
Quartz Crystal Microbalance (QCM) measurements were carried out on
Qsense D300 equipment with a Ti sensor (Q-Sense, Vastra Frolunda) that
was intensively cleaned with a UV-ozone ProCleaner system (BioForce
Nanosciences, Ames, IA) prior to use. Before measurements, the Ti-sensor
and the chamber were equilibrated in 50 mM Tris-HCl (pH 8.0) and 150 mM
NaCl (TBS) containing 0.05% polyoxyethylene sorbitan monolaurate (Tween
20) (TBST) at 25 °C. The proteins were prepared in a 2 mL volume at a
concentration of 0.5 µM in TBST. The data were obtained and analyzed by
Q soft 301 and q-tool (Q-Sense).