2.1 Field sampling
Frogs were sampled between the months of March and July in 2013, 2014,
and 2015. We collected a total of 191 P. auritus from four sites
in Cameroon (Campo Ma’an (CM), N = 29; Ebo forest (EF), N = 24;
Ndikiniméki (ND), N = 13; and Takamanda (TM), N = 15), and five sites in
Gabon (Gamba Complex (GC), N = 24; Kessala (KS), N = 21; Lopé (LP), N =
18; Monts de Cristal (MC), N = 22; and Minkébé (MK), N = 25) (Fig.
1a).These sites encompassed a range of forest types, namely: lowland
rainforest (CM, MK), sub-montane rainforest (EF, TM), forest-savanna
ecotone (KS, MC), coastal rainforest (GC), and mixed
lowland-agricultural forest (ND). After frogs were euthanized with MS222
solution, muscle and kidney tissue was placed in 95% ethanol for DNA
extraction. Male frogs were differentiated from female frogs by the
presence of a developed vocal sac, indicated by a dark throat and vocal
folds. Frogs that could not be sexed by these secondary sexual
characteristics were dissected and sexed by either the type of
reproductive organ or by the presence of eggs. All animal handling
procedures were carried out according to an approved Institutional
Animal Care and Use Committee protocol 12-008. Specimens were deposited
at the Museum of Comparative Zoology, Harvard University.