2.3 RNA Extraction
Total RNA extraction from all clinical samples was conducted using the MagMAX Express-96 Magnetic Particle Processor (Thermo-Fisher) using the MagMax CORE Nucleic Acid Purification Kit (Thermo-Fisher) with some modification. Samples were inactivated with tripure reagent (1:9 ratio). Then, 650 µL of the inactivated sample, 30µL of binding beads, and 350 µL binding buffer spiked with enteroviral armoured RNA (ARM-ENTERO; Asuragen) was used for extraction. The extracted RNA was eluted in 30 μL elution buffer. The spiked enteroviral armoured RNA was used as an exogenous RNA extraction control.