2.3 RNA Extraction
Total RNA extraction from all clinical samples was conducted using the
MagMAX Express-96 Magnetic Particle Processor (Thermo-Fisher) using the
MagMax CORE Nucleic Acid Purification Kit (Thermo-Fisher) with some
modification. Samples were inactivated with tripure reagent (1:9 ratio).
Then, 650 µL of the inactivated sample, 30µL of binding beads, and 350
µL binding buffer spiked with enteroviral armoured RNA (ARM-ENTERO;
Asuragen) was used for extraction. The extracted RNA was eluted in 30 μL
elution buffer. The spiked enteroviral armoured RNA was used as an
exogenous RNA extraction control.