3.5. Agomelatine supplementation enhances endothelial
function
The endothelium-dependent vasodilator response to acetylcholine of
aortic rings was measured to analyse the functionality of the
endothelium. The results showed a reduction in vasodilatory responses in
the HFD group in comparison with control mice (Figure 10A). Improved
endothelial relaxation could be observed in mice treated with the
highest doses of agomelatine, as well as with melatonin and metformin.
Since this could be related to the oxidative stress, the activity of the
enzyme NADPH, the main source of reactive oxygen species in this
location, was measured. Obese mice exhibited a more pronounced
activation than the lean ones. The highest doses of agomelatine,
melatonin and metformin downregulated it (Figure 10B).