2.15 Hair patch assay
Epidermal and dermal cells were isolated from neonatal mice. The skin of
neonatal mice was peeled and digested with 0.25% β-trypsin to separate
the epidermis and dermis, and the separated dermal cells were seeded in
100 mm culture dish and incubated with 5 μM KY19382 or DMSO for 72
hours. 1×106 epidermal and 1.5×106dermal cells were injected subcutaneously into hairless mice.