Genomic DNA extraction
A single colony from a clinical isolate was inoculated in tryptic soy
broth (TSB) for 16 h, and 1 mL of overnight culture was harvested using
centrifugation at 16,500 ×g for 5 min. Bacterial cells were suspended in
1 mL of ultrapure water and heated at 100 °C for 15 min. The supernatant
containing the DNA was stored at 4 °C until further use.