Burkholderia cepacia complex species identification
We conducted recA sequencing to identify Burkholderia
cepacia complex (BCC) species. We performed PCR amplification using
specific primers and conditions described by Fehlberg et al. (13). Cycle
sequencing was performed using a BigDye Terminator v3.1 cycle sequencing
kit and an ABI 3730xl DNA analyzer. We further analyzed the recAsequences and aligned them to a database using NCBI BLASTn.