Study 1. Effect of benralizumab and mepolizumab on the CRC to histamine
Concentration-response curves (CRCs) to histamine were generated in isolated airways. The CRCs were produced via the cumulative administration of histamine. Airways were stimulated with histamine until a stable level of contractility was reached for each concentration, usually for 5-15 min. After that, the next concentration of agonist was administered. Experiments were performed in C-, passively sensitized airways (positive control, C+), and in passively sensitized airways that were incubated overnight with different concentrations of either benralizumab or mepolizumab during the sensitizing procedure.