Quantification of IL-5 and cAMP
The supernatant of C- and C+ tissues was collected at different
time-points during the overnight incubation in the presence of
sensitizing and non-sensitizing sera in order to assess the release of
IL-5 from the bronchial tissue. The concentrations of IL-5 were
quantified by using human IL-5 ELISA kit (RayBiotech, Inc., GA,
USA)
according to manufacturers’ instructions (available at:
https://www.raybiotech.com/files/manual/ELISA/ELH-IL5.pdf).
At the end of experiments, isolated bronchi treated with either
benralizumab or mepolizumab were collected along with C- and C+ airways
in order to quantify the tissue levels of cAMP by using a cAMP ELISA kit
(RayBiotech, Inc., GA,
USA) according to manufacturers’ instructions
(available at:
https://www.raybiotech.com/files/manual/EIA/EIA-CAMP.pdf).