Study 4. Effect of CRCs to benralizumab and mepolizumab on the contractile response to specific EFS frequencies
Isolated airways were stimulated by EFS delivered at frequencies inducing different levels of contractile response to isolated airways that were previously incubated overnight with different concentrations of either benralizumab or mepolizumab during the sensitizing procedure. The EFS frequencies delivered to elicit the specific contractile responses were the half maximal effective frequency (EF50), the 70% effective frequency (EF70), and the 90% effective frequency (EF90) with respect to the Emaxdetectable in C+ isolated bronchi. Each specific EFS frequency was delivered after that the contractile response induced by the previous EFS was terminated, usually in 3-5 min. Experiments were performed also in C- and C+ tissues.