Study 5. Effect of benralizumab and mepolizumab on the contractile response to QS
QS of 0.5 mm was elicited in isolated airways, as previously described (Mitchell et al., 1997). Briefly, a calibrated thumbscrew enabled the accurate measurement of the angular rotation of the threaded rod that was used to induce QS. Since the pitch of the screw was 1.0 mm, a rapid (<200 ms) 180° rotation was performed to elicited a QS of 0.5 mm on the bronchial rings. The resulting myogenic contractile response was recorded for 10 min. QS was performed in C- and C+ tissues, and in passively sensitized airways that were incubated overnight with different concentrations of either benralizumab or mepolizumab during the sensitizing procedure.