Figure legends.
Figure 1. Production of pUC18 in E. coliW3110recA - (left panel) and W3110recA - vgb + (right panel) in fed-batch cultures with a step-change to O2-limited regime. The time profiles of biomass and glucose concentrations (a, e), organic acids and ethanol accumulation (b, f), plasmid DNA (pDNA) production, supercoiled pDNA fraction, and pDNA yield from biomass (c, g), oxygen transfer rate (OTR), carbon dioxide evolution rate (CER) and dissolved oxygen tension (DOT) (d, h) are shown. Error bars show the deviation between duplicates. pDNA, plasmid DNA.
Figure 2. (a) Plasmid copy number per chromosome (PCN), (b) Relative RNAII copies/RNAi copies, normalized to sample at 4 hr, and (c) relative vgb copy number, normalized to sample at 4 hr, in fed-batch culture samples taken at 4, 10 and 12 hr of culture.
Figure 3. Metabolic fluxes estimated by flux balance analysis in fed-batch cultures of W3110recA - (left row) and W3110recA - vgb +(right row) with a step-change to O2-limited regime. Calculations were performed for pseudo steady-states (constant growth rates and yields) during the aerobic batch (top numbers), aerobic phase of the fed-batch (middle numbers) and microaerobic phase of the fed-batch (bottom numbers). All values are given in mmol/g hr.