Co-evolution of expansin domains
For comparison of the co-occurrence of the two expansin domains, all
sequence entries from the ExED were aligned against the two profile HMMs
for the expansin domains. Profile-to-sequence alignments were performed
with the hmmsearch command from the HMMER software suite with themax option to collect all domain-based scores for each possible
alignment. The lists of domain-based scores were sorted by sequence
identifiers to ensure comparability, and in case of multiple hits, only
the maximal bit score was kept. The bivariate histogram was visualized
as heat map for bit scores greater than zero in MATLAB (version R2019a,
The MathWorks, Natick, MA, USA).