Intermittent rivers are prevalent in many countries across Europe and in Mediterranean countries outside Europe, but little is known about the temporal evolution of intermittency characteristics and their relationships with climate variability. In this study, a trend analysis is performed on the annual and seasonal number of zero-flow days, the maximum duration of dry spells and the mean date of the zero-flow events, on a database of 452 rivers in European and in Mediterranean countries outside Europe, with varying degrees of intermittence. In addition, the relationships between flow intermittence and climate are investigated using the Standardized Precipitation Evapotranspiration Index (SPEI) and six climate indices describing large scale atmospheric circulation. Results indicated a strong spatial variability of the seasonal patterns of intermittence and the annual and seasonal number of zero-flow days, which highlights the controls exerted by local catchment properties. Most of the detected trends indicate an increasing number of zero-flow days which also tend to occur earlier in the year, in particular in Southern Europe. The SPEI is found to be strongly related to the annual and seasonal zero-flow day occurrence in more than half of the stations for different accumulation times between 12 and 24 months. Conversely, there is a weak dependence of river intermittence with large-scale circulation indices. Overall, these results suggest increased water stress in intermittent rivers that may affect their biota and biochemistry and also reduce available water resources.