Impacts of antibiotic reagents on morphology and differentiation in Phaseolus vulgaris callus tissue
AbstractThough one of the most common methods for reproducing plants, propagation through seeds is often not feasible because of a consistently low germination percentage. Instead, this project aims to study the viability of using Phaseolus vulgaris (known as the common bean) as a model organism in antibiotic resistance studies. Specifically, this project seeks to investigate the effectiveness of external antibiotics in promoting the growth and differentiation of common bean callus growth. The single experimental group encapsulates bean callus growth medium with added cefotaxime, streptomycin, and kanamycin which are grown in standard growth medium with the addition of these antibiotics. The control group compares green bean callus growth in standard medium. To further evaluate the morphological differences between various mediums, a measure of the dry mass of the callus along with the study of its mitotic index was used to determine the effectiveness of each antibiotic reagent in improving the growth of the callus. Ultimately, the results refute the original hypothesis which predicted that all four antibiotics would have a positive benefit on growth and regeneration of the callus tissue. Rather, when measuring callus health, only kanamycin had a significant effect (Mann-Whitney U = 3.5, p-value = 0.0385) on the growth factors of the callus tissue through its high mitotic index. Future research may apply these findings to focus on computational aspects in studying the effect kanamycin has on somatic embryogenesis via callus growth in an effort to inhibit bacterial growth which reduces the chance of infection in the callus. By utilizing kanamycin resistance as a selectable marker, researchers can easily identify and select transformed plants taken up by foreign DNA and further simplify the study of genetically modified plant species, which has significant implications for the future of agricultural production.