Background: Movement is a contributing factor to the risk of exposure to infectious disease. A primary reason for equine travel in Ontario, Canada is for equestrian competitions. The Canadian National Farm and Facility Level Biosecurity Standard for the Equine Sector provides guidance for equestrian facilities regarding strategies to reduce the risk of exposure to pathogens at competitions and upon return. To be further investigated are the perceptions of these recommendations by facility managers and their degree of implementation. Objectives: The objective of this study was to determine the biosecurity perspectives of equestrian facility managers to be able to identify challenges and opportunities regarding equestrian facility-level biosecurity. Study Design: This study used a qualitative approach to gather in-depth data on the experiences of equestrian facility managers Methods: Individual, semi-structured interviews were conducted in-person with 10 Ontario equestrian facility managers. A template analysis using deductive coding was used to determine themes. Results: The main themes identified were: (i) facility managers feel ultimately primarily responsible for biosecurity choices, but also feel that the overall responsibility should be shared; and (ii) facility managers understand biosecurity in theory but face challenges putting it into practice. Main Limitations: This study asked for voluntary participation, leading to the potential for self-selection bias with participants having a pre-existing interest in biosecurity. Participants residing in specific geographic radius of Toronto, Ontario could also lead to possible similarities between facilities. The small sample size also may result in nonresponse bias. These biases could lead to the sample population not being representative of the general population Conclusions: Results of this study indicate the need for additional focus on overcoming barriers to implementation and bridging the gap between stakeholder groups. This would allow for improvements in equine biosecurity at both home facilities and competitions, positively impacting overall equine health and welfare