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A Comprehensively Dynamic Immune Response Acetylation Network Induced by Pathogen Puccinia polysora in Maize
  • +2
  • Jianfei Guo,
  • Zhigang Ma,
  • Ce Deng,
  • Junqiang Ding,
  • Yuxiao Chang
Jianfei Guo
Shenzhen Branch Guangdong Laboratory of Lingnan Modern Agriculture Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural Sciences Shenzhen 518120 China
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Zhigang Ma
Shenzhen Branch Guangdong Laboratory of Lingnan Modern Agriculture Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural Sciences Shenzhen 518120 China
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Ce Deng
College of Agronomy Henan Agricultural University Zhengzhou 450046 China
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Junqiang Ding
College of Agronomy Henan Agricultural University Zhengzhou 450046 China
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Yuxiao Chang
Shenzhen Branch Guangdong Laboratory of Lingnan Modern Agriculture Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural Sciences Shenzhen 518120 China

Corresponding Author:[email protected]

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Abstract

Lysine-ε-acetylation (Kac) is a reversible post-translational modification that plays important roles during plant-pathogen interactions. Some pathogens can deliver secreted effectors that encode acetyltransferase or deacetylases into host cell directly modify acetylation of host proteins. However, how Kac dynamically affects defense response remains unclear. We describe a highly dynamic Kac catalog from pathogen colonization to symptom visible in maize. Mass spectrometry-based approach was performed to quantify the protein abundance and levels of Kac proteins in southern-corn rust (SCR)-resistant and susceptible maize infected with Puccinia polysora for 0h, 12h, 24h, 48h and 72h. Proteome-wide analysis identified 7412 Kac site from 4697 proteins, in which 1732 Kac sites in 1006 proteins were quantified. Among them 1680 and 7128 Kac sites were novelly found in quantified and identified dataset, respectively. The Kac motif -Y/W/F-X-X-Kac-Y/P/T/S/N- was specifically found in this study. The proteins involved in signaling transduction, defense response, cell wall fortification, ROS scavenging, redox reaction and proteostasis were differentially acetylated, and some of them Kac levels presented converse pattern in SCR-resistant and susceptible maize. Our acetylproteomics not only greatly extending our knowledge of in vivo acetylation during plant-pathogen interactions but also firstly providing a comprehensively dynamic Kac catalog for plant immune response.