Immunization has been considered as a successful global health program that saves life of many persons each year. The vaccines reduce the risk of getting the disease by providing immunity in the body. Therefore, the constant availability of essential vaccines is an important factor in the health of the community. One of the most important vaccines worldwide is the diphtheria vaccine, which is usually given multivalent with tetanus and pertussis. It takes about 45 days to produce this vaccine from the time of initial culture to the production of the toxoid. If it is possible to carry out many stages of production under normal conditions and maintain the chemical intermediate, it will be a great help to produce this vaccine. In this study, the method of purification (by washing and chromatography) and storage of toxins for several months is presented. In this method, by concentrating the sample, it can be stored in a smaller space and there will be no problem in providing a suitable space. Another problem with the diphtheria vaccine is that it is reversible after detoxification of the toxin using formaldehyde. In order to solve this problem, it is suggested to use mPEG for detoxification, which will produce more stable covalent bonds between PEG and the first type of amine groups (-NH2) in the toxin chain. Therefore, we will have a stable product that the results of animal tests show that this product caused no wound or necrosis in the tested animals.