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Comparative study of Scleromitrion diffusum and Oldenlandia corymbosa: mircoscopy, TLC, HPLC and antioxidant activity
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  • Yuan Shiun Chang,
  • Yu-Ling Ho,
  • Hung-Yi Wu,
  • Thanh-Thuy-Dung Au,
  • Kun-Chang Wu
Yuan Shiun Chang
China Medical University Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources

Corresponding Author:[email protected]

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Yu-Ling Ho
HungKuang University
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Hung-Yi Wu
Chugai Pharma Taiwan Ltd
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Thanh-Thuy-Dung Au
China Medical University Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources
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Kun-Chang Wu
China Medical University College of Pharmacy
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Abstract

Quality control of herbal medicines is an important and necessary issue, especially the role of herbal drug identification to avoid misuse of herb that affect efficacy, or cause toxicity. Scleromitrion diffusum (SD) is one of the most common herbs but often confused with Oldenlandia corymbosa (OC). 10 samples of SD and 10 samples of OC were collected on the Taiwan markets. This study conducted the analysis of morphology, microscopy, TLC, HPLC using two markers Asperuloside (ASP) and Scandoside methyl ester (SME) to distinguish SD and OC. Through quantification of total polyphenols and total flavanoids, investigating antioxidant activity through DPPH free radical scavenging effect, ABTS •+ scavenging effect and reducing power to further clarify the biological effects of two herbs. Study results show the differences in microscopy and suggests a TLC method for distinguishing two herbs on the markets. In HPLC, the ratio of two markers ASP and SME in two herbs was also different when SD contained higher content of ASP and conversely OC higher SME. With more total polyphenols and total flavonoids in SD than that in OC, the antioxidant activity of SD is superior to that of OC. This study provides a more comprehensive perspective for identification and quality evaluation of SD on the markets.
20 Jan 2023Submitted to Microscopy Research and Technique
30 Jan 2023Submission Checks Completed
30 Jan 2023Assigned to Editor
30 Jan 2023Review(s) Completed, Editorial Evaluation Pending
30 Jan 2023Reviewer(s) Assigned
06 Feb 2023Editorial Decision: Revise Major
31 Jan 20241st Revision Received
31 Jan 2024Submission Checks Completed
31 Jan 2024Assigned to Editor
31 Jan 2024Review(s) Completed, Editorial Evaluation Pending
22 Mar 2024Submission Checks Completed
22 Mar 2024Assigned to Editor
23 Mar 2024Reviewer(s) Assigned