“RNA-templated/directed DNA repair” is a new biological mechanism that has been experimentally demonstrated in bacteria, yeast and mammalian cells. Different RNAs or artificial DNA/RNA hybrid molecules have been used to study their role in DNA double-strand break (DSB) repair. Recent work also demonstrated that small non-coding RNAs (DDRNAs produced by DICER, Drosha) and/or newly RNAPII transcribed RNA (dilncRNA) are orchestrating the initial steps in DSB repair processes, giving another hint for the involvement of RNA in DNA repair processes. Here we demonstrate that pre-mRNA molecules could be used for DSB repair. Our mamma¬lian cell culture system is based on 3 components: (1) a mutated reporter gene producing an intron-containing pre-mRNA, (2) an sgRNA-guided dCas13b::ADAR RNA editor specific for this pre-mRNA, and (3) I-SceI to create a transient DSB situation. We were able demonstrate that the ADAR-edited pre-mRNA is being used in cis for DSB repair, thereby converting the mutated reporter gene into an active reporter gene due to reconstituted splicing. We also used overexpression and knock-down of several proteins to investigate their putative role in this novel RNA-mediated DNA repair (RmDR) pathway. Based on our data, RNA can be used as template for DNA repair processes.