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Kinetic study of liquid lipase-catalyzed glycerolysis of olive oil using Lipozyme TL 100L
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  • George Finco,
  • Karina Fiametti,
  • E.A. da Salva,
  • Fernando Palú,
  • João Wancura,
  • Alexsandra Valério,
  • Jose Oliveira
George Finco
UNIOESTE Campus de Toledo

Corresponding Author:[email protected]

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Karina Fiametti
Universidade Tecnológica Federal do Paraná - Câmpus Toledo
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E.A. da Salva
Univ. Estadual Do Oeste Do Parana
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Fernando Palú
Universidade Tecnológica Federal do Paraná - Câmpus Toledo
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João Wancura
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Alexsandra Valério
Federal University of Santa Catarina
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Jose Oliveira
Federal University of Santa Catarina
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Abstract

Monoacylglycerol (MAG) and diacylglycerol (DAG) are two natural components found in most edible oils and fats. Conventional synthesis of MAG and DAG is usually conducted by glycerolysis of triacylglycerol (TAG) at high temperatures (above 200 °C) in the presence of an alkaline catalyst. In this work, the synthesis of MAG and DAG using enzymatic glycerolysis of olive oil was investigated using Tween 80 as surfactant, n-butanol as co-surfactant and the novel lipase in free/liquid formulation Lipozyme TL 100L as catalyst. Experimental design was used to evaluate the effect of enzyme load and reaction temperature on the feedstock conversion. Enzyme load and system temperature were significant variables in the statistical design and the best condition was found at 35 °C, 7.5 vol% of Lipozyme TL 100 and glycerol to oil molar ratio of 2:1 with conversion of TAG at approximately 98 % after 2 h of process. A mathematical model based on the Ping-Pong Bi-Bi mechanism was used to describe the reaction kinetics. The model adequately described the behavior of the system and can be a useful tool for the design of reactors in larger scales.
09 Oct 2021Submitted to Journal of the American Oil Chemists' Society
11 Oct 2021Submission Checks Completed
11 Oct 2021Assigned to Editor
11 Oct 2021Reviewer(s) Assigned
04 Nov 2021Review(s) Completed, Editorial Evaluation Pending
08 Nov 2021Editorial Decision: Revise Major
07 Jan 20221st Revision Received
07 Jan 2022Submission Checks Completed
07 Jan 2022Assigned to Editor
02 Mar 2022Reviewer(s) Assigned
19 Mar 2022Review(s) Completed, Editorial Evaluation Pending
28 Mar 2022Editorial Decision: Revise Minor
03 Apr 20222nd Revision Received
04 Apr 2022Submission Checks Completed
04 Apr 2022Assigned to Editor
08 Apr 2022Review(s) Completed, Editorial Evaluation Pending
15 Apr 2022Editorial Decision: Accept
Jul 2022Published in Journal of the American Oil Chemists' Society volume 99 issue 7 on pages 559-568. 10.1002/aocs.12593