To study the genetic diversity and structure of the forest species Pterocarpus erinaceus Poir., seventeen polymorphic nuclear microsatellite markers were isolated and characterized, using Illumina MiSeq sequencing technology. Three hundred and sixty five (365) individuals were analysed within fifteen (15) West Africa populations. The alleles’ number for these loci varied from 4 to 30 and 0.23 to 0.82 for the heterozygosity. The seventeen primers designed here will be useful to analyse ecology population and mechanisms of population differentiation of this threaten species.